Detection of a Cis eQTL Controlling BMCO1 Gene Expression Leads to the Identification of a QTG for Chicken Breast Meat Color - Institut Agro Rennes-Angers Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2011

Detection of a Cis eQTL Controlling BMCO1 Gene Expression Leads to the Identification of a QTG for Chicken Breast Meat Color

Cécile Berri
Frederique Pitel
Benoit Graulet
Estelle Godet
  • Fonction : Auteur
  • PersonId : 1202647
Sophie Y. Leroux
Sandrine Lagarrigue
Cécile Duby
  • Fonction : Auteur
Catherine M. Beaumont
Michel Jacques M.J. Duclos

Résumé

Classical quantitative trait loci (QTL) analysis and gene expression QTL (eQTL) were combined to identify the causal gene (or QTG) underlying a highly significant QTL controlling the variation of breast meat color in a F2 cross between divergent high-growth (HG) and low-growth (LG) chicken lines. Within this meat quality QTL, BCMO1 (Accession number GenBank: AJ271386), encoding the beta-carotene 15, 15'-monooxygenase, a key enzyme in the conversion of beta-carotene into colorless retinal, was a good functional candidate. Analysis of the abundance of BCMO1 mRNA in breast muscle of the HG x LG F2 population allowed for the identification of a strong cis eQTL. Moreover, reevaluation of the color QTL taking BCMO1 mRNA levels as a covariate indicated that BCMO1 mRNA levels entirely explained the variations in meat color. Two fully-linked single nucleotide polymorphisms (SNP) located within the proximal promoter of BCMO1 gene were identified. Haplotype substitution resulted in a marked difference in BCMO1 promoter activity in vitro. The association study in the F2 population revealed a three-fold difference in BCMO1 expression leading to a difference of 1 standard deviation in yellow color between the homozygous birds at this haplotype. This difference in meat yellow color was fully consistent with the difference in carotenoid content (i.e. lutein and zeaxanthin) evidenced between the two alternative haplotypes. A significant association between the haplotype, the level of BCMO1 expression and the yellow color of the meat was also recovered in an unrelated commercial broiler population. The mutation could be of economic importance for poultry production by making possible a gene-assisted selection for color, a determining aspect of meat quality. Moreover, this natural genetic diversity constitutes a new model for the study of beta-carotene metabolism which may act upon diverse biological processes as precursor of the vitamin A.
Fichier principal
Vignette du fichier
journal.pone.0014825_1_.pdf (605.16 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-00729320 , version 1 (03-04-2013)

Identifiants

Citer

Elisabeth Le Bihan-Duval, Javad Nadaf, Cécile Berri, Frederique Pitel, Benoit Graulet, et al.. Detection of a Cis eQTL Controlling BMCO1 Gene Expression Leads to the Identification of a QTG for Chicken Breast Meat Color. PLoS ONE, 2011, 6 (7), non paginé. ⟨10.1371/journal.pone.0014825⟩. ⟨hal-00729320⟩
341 Consultations
266 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More