Arabidopsis thaliana cells: a model to evaluate the virulence of Pectobacterium carotovorum.

Meriam Terta 1 M. Kettani-Halabi 1, 2 Khadija Ibenyassine 1, 2 Daniel Tran 1 Patrice Meimoun 1 Rajae M'Hand 2 Hayat El-Maarouf-Bouteau 3 Florence Val 4, * Mohamed Mustapha Ennaji 2 François Bouteau 1
* Corresponding author
1 Laboratoire d'Electrophysiologie des Membranes (LEM) EA 3514
2 LVHM - Laboratoire de virologie et Hygiène et Microbiologie
3 UR5-UPM
4 UMR BIO3p
BIO3P - Biologie des organismes et des populations appliquées à la protection des plantes
Abstract : Pectobacterium carotovorum are economically important plant pathogens that cause plant soft rot. These enterobacteria display high diversity world-wide. Their pathogenesis depends on production and secretion of virulence factors such as plant cell wall-degrading enzymes, type III effectors, a necrosis-inducing protein, and a secreted virulence factor from Xanthomonas spp., which are tightly regulated by quorum sensing. Pectobacterium carotovorum also present pathogen-associated molecular patterns that could participate in their pathogenicity. In this study, by using suspension cells of Arabidopsis thaliana, we correlate plant cell death and pectate lyase activities during coinfection with different P. carotovorum strains. When comparing soft rot symptoms induced on potato slices with pectate lyase activities and plant cell death observed during coculture with Arabidopsis thaliana cells, the order of strain virulence was found to be the same. Therefore, Arabidopsis thaliana cells could be an alternative tool to evaluate rapidly and efficiently the virulence of different P. carotovorum strains.
Keywords : erwinia-carotovora subsp carotovora potato diseases plant pathogen identification chrysanthemi atroseptica diversity responses strains
Document type :
Journal articles
Complete list of metadatas

Cited literature [26 references]  Display  Hide  Download
https://hal-agrocampus-ouest.archives-ouvertes.fr/hal-00729445
Contributor : Céline Martel <>
Submitted on : Tuesday, March 5, 2013 - 4:31:32 PM
Last modification on : Thursday, April 4, 2019 - 1:27:19 AM
Long-term archiving on : Thursday, June 6, 2013 - 3:55:15 AM

File

MPMI-23-2-0139.pdf
Publisher files allowed on an open archive

Identifiers

Collections

UNAM | AGROCAMPUS-OUEST | UNIV-RENNES1 | UPMC | INRA | UMR-IGEPP-INSTITUT-DE-GENETIQUE-ENVIRONNEMENT-ET-PROTECTION-DES-PLANTES-AGROCAMPUS-OUEST | UNIV-RENNES | SORBONNE-UNIVERSITE | UNIV-PARIS7 | USPC

Citation

Meriam Terta, M. Kettani-Halabi, Khadija Ibenyassine, Daniel Tran, Patrice Meimoun, et al.. Arabidopsis thaliana cells: a model to evaluate the virulence of Pectobacterium carotovorum.. Molecular Plant-Microbe Interactions, American Phytopathological Society, 2010, 23 (2), pp.139-43. ⟨10.1094/MPMI-23-2-0139⟩. ⟨hal-00729445⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

438

Files downloads

760

CONTACT : publication@agrocampus-ouest.fr