Molecular characterization of the sucrose transporter HbSUT6 in relation with rubber production in laticiferous cells

Abstract : The rubber synthesis in latex cells requires sucrose. These particular cells are heterotrophic ones and are apoplastically connected to other tissues. Such features imply that the rubber yield may depend on the latex cell efficiency to absorb and to use the imported sucrose. This study investigates the sucrose transporters as key genes for rubber biosynthesis involved in the ethylene-stimulated rubber yield. Seven putative sucrose transporters had been isolated from a latex-derived cDNA library of PB 217. Expression profiling of these sucrose transporters under ethylene stimulation in PB 217 underlined the role of one sucrose transporter: HbSUT1B (Hevea brasiliensis Sucrose Transporter 6) (Dusotoit-Coucaud et al., 2007). This paper reports the next stage in HbSUT1B, molecular characterisation. Its expression in different parts of the rubber tree was studied. It was preferentially expressed in leaves and bark. This result was completed by in-situ hybridisation experiments. In addition, promoter cloning and analysis revealed some clues for the wounding regulation of HbSUT1B. The HbSUT1B expression was analysed in response to different treatments in latex or bark tissues. HbSUT1B was considerably up-regulated by hormones and wounding in bark but not in latex. This regulation appeared to be tissue-specific.
Type de document :
Communication dans un congrès
International Rubber Resarch Development Board Congress (IRRDB), Oct 2008, Kuala Lumpur (MAL), Malaysia
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https://hal-agrocampus-ouest.archives-ouvertes.fr/hal-00729771
Contributeur : Céline Martel <>
Soumis le : vendredi 7 septembre 2012 - 15:54:14
Dernière modification le : mercredi 21 mars 2018 - 16:08:10

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  • HAL Id : hal-00729771, version 1

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Anaïs Dusotoit-Coucaud, N. Brunel, N. Franchel, N. Granet, H. Chrestin, et al.. Molecular characterization of the sucrose transporter HbSUT6 in relation with rubber production in laticiferous cells. International Rubber Resarch Development Board Congress (IRRDB), Oct 2008, Kuala Lumpur (MAL), Malaysia. 〈hal-00729771〉

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