Assessment of the genetic diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp fuscans as a basis to identify putative pathogenicity genes and a type III secretion system of the SPI-1 family by multiple suppression subtractive hybridisations

Seyed Mehdi Alavi; Seyed Sanjari; Fabien Durand; Christelle Brin; Charles Manceau; Stéphane Poussier

Assessment of the genetic diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp fuscans as a basis to identify putative pathogenicity genes and a type III secretion system of the SPI-1 family by multiple suppression subtractive hybridisations

Seyed Mehdi Alavi ¹ Seyed Sanjari ¹ Fabien Durand ¹ Christelle Brin ¹ Charles Manceau ¹ Stéphane Poussier ^{2, *}

* Corresponding author

1 Pavé

PaVé - Pathologie Végétale

2 PaVé - Pathologie Végétale

Abstract : Fluorescent amplified fragment length polymorphism revealed that strains of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans are genetically distinct and can be grouped into four genetic lineages. Four suppression subtractive hybridizations were then performed to isolate DNA fragments present in these bean pathogens and absent from closely related xanthomonads. Virulence gene candidates were identified such as homologs of hemagglutinins, TonB-dependent receptors, zinc-dependent metalloproteases, type III effectors, and type IV secretion system components. Unexpectedly, homologs of the type III secretion apparatus components (SPI-1 family), usually reported in animal pathogens and insect symbionts, were also detected.

Mots-clés : XANTHOMONAS AXONOPODIS XANTHOMONAS FUSCANS PATHOGENICITE GENES DE VIRULENCE BACTERIES PHYTOPATHOGENES HYBRIDATION SUPPRESSIVE SOUSTRACTIVE DIVERSITE GENETIQUE

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Vegetal Biology / Phytopathology and phytopharmacy

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https://hal-agrocampus-ouest.archives-ouvertes.fr/hal-00729841
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Submitted on : Tuesday, March 12, 2013 - 4:36:34 PM
Last modification on : Thursday, April 11, 2019 - 12:34:26 PM
Long-term archiving on : Monday, June 17, 2013 - 11:04:58 AM

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Seyed Mehdi Alavi, Seyed Sanjari, Fabien Durand, Christelle Brin, Charles Manceau, et al.. Assessment of the genetic diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp fuscans as a basis to identify putative pathogenicity genes and a type III secretion system of the SPI-1 family by multiple suppression subtractive hybridisations. Applied and Environmental Microbiology, American Society for Microbiology, 2008, 74 (10), pp.3295-3301. ⟨10.1128/AEM.02507-07⟩. ⟨hal-00729841⟩

Assessment of the genetic diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp fuscans as a basis to identify putative pathogenicity genes and a type III secretion system of the SPI-1 family by multiple suppression subtractive hybridisations

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Assessment of the genetic diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp fuscans as a basis to identify putative pathogenicity genes and a type III secretion system of the SPI-1 family by multiple suppression subtractive hybridisations

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