Assignment of Aegilops variabilis Eig chromosomes and translocations carrying resistance to nematodes in wheat.

Abstract : The allotetraploid species Aegilops variabilis Eig (2n = 28, UUSvSv) belongs to the tribe Triticeae and is closely related to wheat. One accession, Ae. variabilis No. 1, was found to be resistant to the cereal cyst nematode (CCN) and the root-knot nematode (RKN). As the genetic variability for resistance to those two pests is limited within wheat, this accession was crossed to bread wheat. Previous work enabled the development of two addition lines and two translocation lines carrying resistance. Here, we demonstrate, using genomic in situ hybridization, that there is no U-Sv interchange in the parental accession of Ae. variabilis. However, there are multiple rearrangements in the Sv chromosomes. The Ae. variabilis chromosome carrying the CreX gene for resistance to CCN combined segments with homoeology to wheat groups 1, 2, 4, and 6. The CreX gene belongs to the group 1 part and it was likely to have been introduced into chromosome 1BL at a similar location as the previously found QTL QCre.srd-1B for CCN resistance. The second Ae. variabilis chromosome carrying CreY and Rkn2 combined segments with homoeology to wheat groups 2, 4, and 7 on its short arm and group 3 on its long arm. It was designated as 3Sv. The two genes for resistance are carried by its long arm and have been transferred to wheat chromosome 3BL through homoeologous and genetically balanced recombination. Different SSR markers present in the introgressed segments could be used in marker-assisted selection.
Type de document :
Article dans une revue
Genome, NRC Research Press, 2009, 52 (4), pp.338-46. 〈10.1139/g09-011〉
Liste complète des métadonnées

https://hal-agrocampus-ouest.archives-ouvertes.fr/hal-00729960
Contributeur : Céline Martel <>
Soumis le : vendredi 7 septembre 2012 - 16:04:05
Dernière modification le : mercredi 16 mai 2018 - 11:23:35

Identifiants

Citation

O. Coriton, Dominique Barloy, V. Huteau, J. Lemoine, A.M. Tanguy, et al.. Assignment of Aegilops variabilis Eig chromosomes and translocations carrying resistance to nematodes in wheat.. Genome, NRC Research Press, 2009, 52 (4), pp.338-46. 〈10.1139/g09-011〉. 〈hal-00729960〉

Partager

Métriques

Consultations de la notice

85