Oculimacula yallundae lifestyle revisited: relationships between the timing of eyespot symptom appearance, the development of the pathogen and the responses of infected partially resistant wheat plants

Abstract : Possible relationships between the timing of eyespot symptom appearance, the development of Oculimacula yallundae and the responses of infected wheat plants were investigated. By comparing, for 45 days after inoculation (d.a.i.), eyespot- and mock-inoculated coleoptiles and leaf sheaths of a wheat line carrying the Pch1 partial resistance gene, this study showed that cell death, used as a criterion for symptom appearance, occurred on the first leaf sheath around 30 d.a.i. Monitoring of O. yallundae development by optical and scanning electron microscopy revealed that the fungus colonized the coleoptile within 30 d.a.i. It then attacked the first leaf sheath and underwent morphological and growth modifications. For 30 d.a.i., infected plants responded by local accumulations of H(2)O(2) and callose in coleoptile epidermal cells and by up-regulation of pathogenesis-related (PR) genes. From 30 d.a.i., natural browning occurred in the first leaf sheath epidermis and the expression level of PR genes, determined by quantitative PCR, significantly increased in infected plants. These results show that the wheat/O. yallundae interaction is characterized by two distinct phases: the asymptomatic phase and the symptomatic phase. The possibility that O. yallundae should be considered hemibiotrophic rather than necrotrophic is discussed.
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Plant Pathology, Wiley, 2009, 58 (1), pp.1-11. 〈10.1111/j.1365-3059.2008.01940.x〉
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Soumis le : vendredi 7 septembre 2012 - 16:06:59
Dernière modification le : mercredi 16 mai 2018 - 11:23:35

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M. Blein, A. Levrel, J. Lemoine, V. Gautier, M. Chevalier, et al.. Oculimacula yallundae lifestyle revisited: relationships between the timing of eyespot symptom appearance, the development of the pathogen and the responses of infected partially resistant wheat plants. Plant Pathology, Wiley, 2009, 58 (1), pp.1-11. 〈10.1111/j.1365-3059.2008.01940.x〉. 〈hal-00730032〉

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