Polymorphic ITS as a tool to identify hybrids and their parents in cultivated genisteae (Fabaceae)

Abstract : Internal Transcribed Spacer (ITS) of ribosomal DNA is a classical sequence used for phylogenetic analyses. Usually, concerted evolution homogenizes the numerous ITS of an organism in such a way that a single sequence is amplified. However, for vegetatively propagated plants of hybrid origin, several ITS sequences can be obtained from a single individual. By cloning ITS sequences of various taxa assigned to tribe Genisteae within Fabaceae, we have been able to show such ITS polymorphism in Cytisus x kewensis, C. x dallimorei, C. x praecox, Genista x spachiana and G. 'Porlock' among others. For these taxa, the distinct ITS sequences can be compared with sequences of putative parents found in literature. We have then confirmed that Cytisus x kewensis is a C. ardoinoi x C. multiflorus cross. We have also confirmed that Genista 'Porlock' is a cross between G.monspessulana and G. x spachiana. Genista x spachiana, commonly presented as G. canariensis x G. stenopetala cross may in fact be a more complex hybrid involving also G. monspessulana.
Type de document :
Communication dans un congrès
XXIII International Eucarpia Symposium, Section Ornamentals: Colourful Breeding and Genetics, Aug 2009, Louvain (BE), Belgium. ISHS - International Society for Horticultural Science, 836, pp.91-96, 2009
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https://hal-agrocampus-ouest.archives-ouvertes.fr/hal-00730050
Contributeur : Céline Martel <>
Soumis le : vendredi 7 septembre 2012 - 16:07:01
Dernière modification le : mercredi 21 mars 2018 - 16:08:09

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  • HAL Id : hal-00730050, version 1

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Valéry Malécot, N. Macquaire-Le Pocreau, G. Auvray, V. Kapusta. Polymorphic ITS as a tool to identify hybrids and their parents in cultivated genisteae (Fabaceae). XXIII International Eucarpia Symposium, Section Ornamentals: Colourful Breeding and Genetics, Aug 2009, Louvain (BE), Belgium. ISHS - International Society for Horticultural Science, 836, pp.91-96, 2009. 〈hal-00730050〉

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