Polymorphic ITS as a tool to identify hybrids and their parents in cultivated Genisteae (Fabaceae)

Abstract : Internal Transcribed Spacer (ITS) of ribosomal DNA is a classical sequence used for phylogenetic analyses. Usually, concerted evolution homogenizes the numerous ITS of an organism in such a way that a single sequence is amplified. However, for vegetatively propagated plants of hybrid origin, several ITS sequences can be obtained from a single individual. By cloning ITS sequences of various taxa assigned to tribe Genisteae within Fabaceae, we have been able to show such ITS polymorphism in Cytisus × kewensis, C. × dallimorei, C. × praecox, Genista × spachiana and G. 'Porlock' among others. For these taxa, the distinct ITS sequences can be compared with sequences of putative parents found in literature. We have then confirmed that Cytisus × kewensis is a C. ardoinoi × C. multiflorus cross. We have also confirmed that Genista 'Porlock' is a cross between G. monspessulana and G. × spachiana. Genista × spachiana, commonly presented as G. canariensis × G. stenopetala cross may in fact be a more complex hybrid involving also G. monspessulana
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Article dans une revue
Acta Horticulturae, International Society for Horticultural Science, 2009, pp.91-96
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https://hal-agrocampus-ouest.archives-ouvertes.fr/hal-00730051
Contributeur : Céline Martel <>
Soumis le : vendredi 7 septembre 2012 - 16:07:01
Dernière modification le : mercredi 21 mars 2018 - 16:08:09

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  • HAL Id : hal-00730051, version 1

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Valéry Malécot, N. Macquaire-Le Pocreau, G. Auvray, V. Kapusta. Polymorphic ITS as a tool to identify hybrids and their parents in cultivated Genisteae (Fabaceae). Acta Horticulturae, International Society for Horticultural Science, 2009, pp.91-96. 〈hal-00730051〉

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