The Induction of Mycotoxins by Trichothecene Producing Fusarium Species

R. Lowe Mélanie Jubault 1 G. Canning Martin Urban K.E. Hammond-Kosack
1 IGEPP - Institut de Génétique, Environnement et Protection des Plantes
Abstract : In recent years, many Fusarium species have emerged which now threaten the productivity and safety of small grain cereal crops worldwide. During floral infection and post-harvest on stored grains the Fusarium hyphae produce various types of harmful mycotoxins which subsequently contaminate food and feed products. This article focuses specifically on the induction and production of the type B sesquiterpenoid trichothecene mycotoxins. Methods are described which permit in liquid culture the small or large scale production and detection of deoxynivalenol (DON) and its various acetylated derivatives. A wheat (Triticum aestivum L.) ear inoculation assay is also explained which allows the direct comparison of mycotoxin production by species, chemotypes and strains with different growth rates and/or disease-causing abilities. Each of these methods is robust and can be used for either detailed time-course studies or end-point analyses. Various analytical methods are available to quantify the levels of DON, 3A-DON and 15A-DON. Some criteria to be considered when making selections between the different analytical methods available are briefly discussed.
Keywords : Chemotype Deoxynivalenol DON Fusarium culmorum Fusarium graminearum Gibberella zeae Head scab disease Induction of mycotoxin production in liquid culture Plant Pathology Plant Sciences Trichothecene mycotoxin detection Wheat ear inoculation
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Submitted on : Tuesday, July 30, 2013 - 2:27:26 PM
Last modification on : Wednesday, June 19, 2019 - 5:17:21 PM

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R. Lowe, Mélanie Jubault, G. Canning, Martin Urban, K.E. Hammond-Kosack. The Induction of Mycotoxins by Trichothecene Producing Fusarium Species. Methods in Molecular Biology, Humana Press, pp.439-455, 2012, ⟨10.1007/978-1-61779-501-5_27⟩. ⟨hal-00849218⟩

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