HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Maternal omega-3 PUFA supplementation prevents hyperoxia-induced pulmonary hypertension in the offspring

Abstract : Pulmonary hypertension (PH) and right ventricular hypertrophy (RVH) affect 16-25% of premature infants with bronchopulmonary dysplasia (BPD), contributing significantly to perinatal morbidity and mortality. Omega-3 polyunsaturated fatty acids (PUFA omega-3) can improve vascular remodeling, angiogenesis, and inflammation under pathophysiological conditions. However, the effects of PUFA omega-3 supplementation in BPD-associated PH are unknown. The present study aimed to evaluate the effects of PUFA omega-3 on pulmonary vascular remodeling, angiogenesis, and inflammatory response in a hyperoxia-induced rat model of PH. From embryonic day 15, pregnant Sprague-Dawley rats were supplemented daily with PUFA omega-3, PUFA omega-6, or normal saline (0.2 ml/day). After birth, pups were pooled, assigned as 12 per litter, randomly assigned to either air or continuous oxygen exposure (fraction of inspired oxygen = 85%) for 20 days, and then euthanized for pulmonary hemodynamic and morphometric analysis. We found that PUFA omega-3 supplementation improved survival, decreased right ventricular systolic pressure and RVH caused by hyperoxia, and significantly improved alveolarization, vascular remodeling, and vascular density. PUFA omega-3 supplementation produced a higher level of total omega-3 in lung tissue and breast milk and was found to reverse the reduced levels of VEGFA, VEGF receptor 2, angiopoietin-1 (ANGPT1), endothelial TEK tyrosine kinase, endothelial nitric oxide synthase, and nitric oxide concentrations in lung tissue and the increased ANGPT2 levels in hyperoxia-exposed rats. The beneficial effects of PUFA omega-3 in improving lung injuries were also associated with an inhibition of leukocyte infiltration and reduced expression of the proinflammatory cytokines IL-1 beta, IL-6, and TNF-alpha. These data indicate that maternal PUFA omega-3 supplementation strategies could effectively protect against infant PH induced by hyperoxia.
Complete list of metadata

https://hal-agrocampus-ouest.archives-ouvertes.fr/hal-01884844
Contributor : Catherine Cliquet Connect in order to contact the contributor
Submitted on : Monday, October 1, 2018 - 2:17:00 PM
Last modification on : Wednesday, April 6, 2022 - 4:08:20 PM

Links full text

Identifiers

Citation

Ying Zhong, Daniel Catheline, Ali Houeijeh, Dyuti Sharma, Lizhong Du, et al.. Maternal omega-3 PUFA supplementation prevents hyperoxia-induced pulmonary hypertension in the offspring. American Journal of Physiology - Lung Cellular and Molecular Physiology, American Physiological Society, 2018, 315 (1), pp.L116 - L132. ⟨10.1152/ajplung.00527.2017⟩. ⟨hal-01884844⟩

Share

Metrics

Record views

44