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A. Pellizaro, M. Neveu, D. Lalanne, . Ly, B. Vu et al., A role for auxin signaling in the acquisition of longevity during seed maturation, New Phytologist, 2019.

, VENUS and DII::VENUS activity in Arabidopsis seeds during maturation. Desiccation tolerance was determined by rapidly drying seeds under an airflow at 43% RH and counting the seeds that germinated after imbibition. Longevity was determined from the survival curves obtained during storage at 75% RH and 35°C (Supporting Informaiton Fig. S1b) and quantified as P50, the storage time needed for half of the seed population to lose viability. (c, d) Activation of DR5 (c) and TIR1 (d) expression in 18-d old seeds by incubation in 1 µM IAA for 4h. (e) Transcript levels of TIR1 during seed maturation. The inset shows the significant increase in the transcript level at 18 and 20 DAP compared to 16 DAP. (f, g) Viability of mature Col-0 (f) and Ler (g) seeds after 21d of aging at 75% RH and 35°C from 10-12 d old siliques that were dipped in 10 µM IAA or mock solution. (h) Viability of mature seeds after 21d of aging at 75% RH and 35°C that were imbibed with or without 1 µM IAA. Significance (student t-test) is indicated by asterisks, Figure 1. Auxin activity correlates with the acquisition of longevity during late seed maturation of Arabidopsis thaliana. (a) Representative images of Col-0 seeds harvested at 12, 14, 16, 18 and 20 days after flowering (DAF). (b) Evolution of desiccation tolerance (DT), longevity (P50) and DR5::GUS, vol.5

, Longevity (expressed as P50) of mature Col-0 and mutant lines, determined from survival curves obtained from storage at 75% RH and 35°C (Supporting Information Fig. S5). (a) cyp79b2-1 and cyp79b2-2, (b) ami1-1 and ami1-2, (c) taa1-1, taa1-2, tar1, tar2, yuc1 and yuc2. Data are means ±S.E.M of 150-200 seeds of three independent biological replicates. Asterisks indicate a significant difference from Col-0 using ANOVA followed by Multiple Comparisons versus Control, Auxin biosynthesis mutants of A. thaliana provide a link between auxin signaling activity and longevity

, (e) ami1-2, (f) taa1-1, tar1, tar2 and yuc2. Data are the mean ± S.E.M of four biological replicates of 100 seeds. (g) Relation between of DR5::GUS activity and longevity (P50) of developing Col-0 seeds (black symbols) and auxin biosynthesis mutant lines (colored symbols). The curve follows a third-order sigmoidal fit. (h) Longevity (expressed as P50) of mature Col-0 and double mutant wei8,tar1. (d-h) Significance (student t-test) is indicated by asterisks, GUS activity in mature seeds of Col-0 and (d), vol.5, pp.79-81

A. Pellizaro, M. Neveu, D. Lalanne, . Ly, B. Vu et al., A role for auxin signaling in the acquisition of longevity during seed maturation, New Phytologist, 2019.