Induced Systemic Resistance (ISR) in Arabidopsis thaliana by Bacillus amyloliquefaciens and Trichoderma harzianum Used as Seed Treatments

Barakat Ilham (1) , Chtaina Noureddine (1) , Philippe Grappin (2) , El Guilli Mohammed (3) , Ezzahiri Brahim (1) , Aligon Sophie (2) , Neveu Martine (2) , Marchi Muriel (2)

1 IAV Hassan II - Institut Agronomique et Vétérinaire Hassan II
2 IRHS - Institut de Recherche en Horticulture et Semences
3 Faculté des Sciences [Kenitra]

The Trichoderma fungal species and the bacteria Bacillus species were described as inducers of plant systemic resistance in relation to their antagonistic activity. The objective of this study was to evaluate the effect of selected strains of Bacillus amyloliquefaciens (I3) and Trichoderma harzianum (A) on inducing systemic resistance in Arabidopsis thaliana as a model for plant molecular genetics. The microorganisms were identified and were confirmed for their antagonistic potential in vitro and in vivo in previous studies. In order to explore this mechanism, two mutants of A. thaliana carrying a PR1 promoter (a conventional marker of salicylic acid (SA) pathway) and LOX2 promoter (a marker triggering jasmonic acid (JA) pathway activation) were analyzed after inoculating antagonists. Transgenic reporter line analysis demonstrated that B. amyloliquefaciens I3 and T. harzianum A induce A. thaliana defense pathways by activating SA and JA at a high level compared to lines treated with chemical elicitors of references (acibenzolar-S-methyl (Bion 50 WG (water-dispersible granule)), SA, and methyl jasmonate). The efficacy of B. amyloliquefaciens I3 and T. harzianum A in inducing the defense mechanism in A. thaliana was demonstrated in this study.

Mots clés: Arabidopsis thaliana; jasmonic acid; salicylic acid; seed treatment; Bacillus amyloliquefaciens I3; Trichoderma harzianum A

Domaines: Sciences et techniques de l'agriculture; Amélioration des plantes

Barakat Ilham, Chtaina Noureddine, Philippe Grappin, El Guilli Mohammed, Ezzahiri Brahim, et al.. Induced Systemic Resistance (ISR) in Arabidopsis thaliana by Bacillus amyloliquefaciens and Trichoderma harzianum Used as Seed Treatments. Agriculture, 2019, 9 (8), pp.166. ⟨10.3390/agriculture9080166⟩. ⟨hal-02277415⟩